| Title | [Study on enzyme-solvent extraction process of flavonoids from leaves of Ginkgo biloba] | | Author(s) | Ouyang NN, Li XZ, Luo Z | | Institution | Materials Science and Engineering Institute, Central South University of Forestry and Technology, Changsha 410004, China. oynn@netease.com | | Source | Zhong Yao Cai 2009 Feb; 32(2):279-83. | | Abstract | OBJECTIVE: To study the optimum enzyme-solvent extraction conditions of flavonoids from leaves of Ginkgo biloba.
METHODS: To optimize the process by single factor experiments and orthogonal test according to the yield of flavonoids.
RESULTS: The optimum extraction conditions were established as follows: pH 5.7, the concentration of enzyme 0.4 mg/mL, enzymatic hydrolysis temperature 45 degrees C, enzymatic hydrolysis time 2 h, the rate of liquid to material 20:1, extraction temperature 70 degrees C, extraction time 2 h, the concentration of ethanol 70%, the average yield of flavonoids from leaves of Ginkgo biloba was 3.62%.
CONCLUSION: The method is simple and can increase material utility effectively. | | Language | chi | | Pub Type(s) | English Abstract
Journal Article
| | PubMed ID | 19504975 |
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